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外泌体是一种纳米级的膜性囊泡,其来源广泛、内容物丰富,在体内参与细胞间通讯、细胞迁移和抗肿瘤免疫等生理过程,与神经疾病和癌症等多种疾病的发生和发展密切相关。外泌体在疾病的精准调控和靶向治疗领域具有光明的应用前景,一方面可以作为疾病的特异性生物标志物,可用于监测疾病进展,治疗反应等。另一方面外泌体具有能够突破血脑屏障、穿越细胞质膜等性质,有望作为潜在的药物载体用于临床治疗。近年来,外泌体已成为新兴的热门研究课题。该文就外泌体在疾病诊断、治疗和药物递送等方面的最新研究进展进行综述,旨在进一步推动外泌体的基础应用研究及临床转化。
Abstract:Exosomes are small, membrane-enclosed vesicles that can originate from a variety of sources and contain a wealth of material. Involved in physiological processes such as cell-cell communication, cell migration and anti-tumor immunity, exosomes are linked to the emergence and progression of various diseases, including neurological disorders and cancer. Exosomes have great potential in the area of precise control and targeted treatment of medical conditions. By serving as a unique biomarker of the disease, they can be utilized to observe the disease's evolution and the response to treatment. On the other hand, exosomes have the potential to be used as drug carriers in clinical treatment due to their ability to break through the blood-brain barrier and pass through the cell plasma membrane. In recent years, exosomes have become an emerging hot research topic. The purpose of this review is to promote the basic research and clinical transformation of exosomes by highlighting the latest developments in exosome-related disease diagnosis, treatment, and drug delivery.
[1]Lebleu V S,Kalluri R.Exosomes as a multicomponent biomarker platform in cancer[J].Trends in Cancer,2020,6(9):767-774.
[2]He J,Ren W,Wang W,et al.Exosomal targeting and its potential clinical application[J].Drug Delivery and Translational Research,2022,12(10):2385-2402.
[3]Zhou M,Li Y J,Tang Y C,et al.Apoptotic bodies for advanced drug delivery and therapy[J].Journal of Controlled Release,2022,351:394-406.
[4]Khalyfa A,Gozal D,Chan W C,et al.Abstract12380:Circulating plasma exosomes in obstructive sleep apnea and reverse-dipping blood pressure[J].Circulation,2018,138:A12380.
[5]Wang W,Luo J,Wang S.Recent progress in isolation and detection of extracellular vesicles for cancer diagnostics[J].Advanced Healthcare Materials,2018,7(20):1800484.
[6]Li P,Kaslan M,Lee S H,et al.Progress in exosome isolation techniques[J].Theranostics,2017,7(3):789-804.
[7]Shu S L,Yang Y,Allen C L,et al.Purity and yield of melanoma exosomes are dependent on isolation method[J].Journal of Extracellular Vesicles,2020,9(1):1692401.
[8]Yang D,Zhang W,Zhang H,et al.Progress,opportunity,and perspective on exosome isolationefforts for efficient exosome-based theranostics[J].Theranostics,2020,10(8):3684-3707.
[9]Zhang Y,Bi J,Huang J,et al.Exosome:A review of its classification,isolation techniques,storage,diagnostic and targeted therapy applications[J].International Journal of Nanomedicine,2020,15:6917-6934.
[10]Chen W,Gan Z,Qin J.Microfluidic strategies for separation and analysis of circulating exosomes[J].Chinese Journal of Chromatography,2021,39(9):968-980.
[11]Gandham S,Su X,Wood J,et al.Technologies and standardization in research on extracellular vesicles[J].Trends in Biotechnology,2020,38(10):1066-1098.
[12]Zhang X,Meng H,Liu H,et al.Advances in laboratory detection methods and technology application of SARS-CoV-2[J].Journal of Medical Virology,2022,94(4):1357-1365.
[13]Hosseini K,Ranjbar M,Pirpour Tazehkand A,et al.Evaluation of exosomal non-coding RNAs in cancer using high-throughput sequencing[J].Journal of Translational Medicine,2022,20(1):3 0.
[14]Xie M,Wang F,Yang J,et al.DNA zipper mediated membrane fusion for rapid exosomal miRNA detection[J].Analytical Chemistry,2022,94(38):13043-13051.
[15]Wang Y,Yang M,Shi H,et al.Photoelectrochemical detection of exosomal miRNAs by combining targetprogrammed controllable signal quenching engineering[J].Analytical Chemistry,2022,94(7):3082-3090.
[16]Zhang Y,Yang F,Wei W,et al.Self-propelled janus mesoporous micromotor for enhanced microRNA capture and amplified detection in complex biological samples[J].ACS Nano,2022,16(4):5587-5 5 96.
[17]Zhand S,Zhu Y,Nazari H,et al.Thiolate DNAzymes on gold nanoparticles for isothermal amplification and detection of mesothelioma-derived exosomal PD-L1mRNA[J].Analytical Chemistry,2023,95(6):3228-3237.
[18]Wang P,Li H,Nie Y,et al.3D Plasmonic nano structure-based polarized ECL sensor for exo some detection in tumor microenvironment[J].ACS Sensors,2023,8(4):1782-1791.
[19]Mukherjee A,Bisht B,Dutta S,et al.Current advances in the use of exosomes,liposomes,and bioengineered hybrid nanovesicles in cancer detection and therapy[J].Acta Pharmacologica Sinica,2022,43(11):2759-2776.
[20]Krainer G,Saar K L,Arter W E,et al.Direct digital sensing of protein bio markers in solution[J].Nature Communications,2023,14(1):653.
[21]Chinnappan R,Ramadan Q,Zourob M.An integrated lab-on-a-chip platform for pre-concentration and detection of colorectal cancer exosomes using antiCD63 aptamer as a recognition element[J].Biosensors and Bioelectronics,2023,220:114856.
[22]Li P,Chen J,Chen Y,et al.Construction of exosome SORL1 detection platform based on 3D porous microfluidic chip and its application in early diagnosis of colorectal cancer[J].Small,2023,19(20):2207381.
[23]Ding L,Liu L-E,He L,et al.Magnetic-nanowaxberrybased simultaneous detection of exosome and exosomal proteins for the intelligent diagnosis of cancer[J].Analytical Chemistry,2021,93(45):15200-15208.
[24]Chavda V P,Pandya A,Kumar L,et al.Exosome nanovesicles:A potential carrier for therapeutic delivery[J].Nano Today,2023,49:101771.
[25]Mihály J,Deák R,SzigyártóI C,et al.Characterization of extracellular vesicles by IR spectroscopy:Fast and simple classification based on amide and CH stretching vibrations[J].Biochimica et Biophysica Acta(BBA)-Biomembranes,2017,1859(3):459-466.
[26]Byeon S K,Lee J Y,Lee J S,et al.Lipidomic profiling of plasma and urine from patients with Gaucher disease during enzyme replacement therapy by nano flow liquid chromatography-tandem mass spectrometry[J].Journal of Chromatography A,2015,1381:132-139.
[27]Jalaludin I,Nguyen H Q,Jang K S,et al.Matrixas sisted laser desorption/ionization-Fourier-transform ion cyclotron resonance-mass spectrometry analysis of exosomal lipids from human serum[J].Rapid Communications in Mass Spectrometry,2023,37(2):e9427.
[28]Sancho-Albero M,Jarne C,Savirón M,et al.Highperformanc e thin-layer chro matography-densito metrytandem ESI-MS to evaluate phospholipid content in exosomes of cancer cells[J].International Journal of Molecular Sciences,2022,23(3):1150.
[29]Elmallah M I Y,Ortega-Deballon P,Hermite L,et al.Lipidomic profiling of exosomes from colorectalcancer cells and patients reveals potential biomarkers[J].Molecular Oncology,2022,16(14):2710-2718.
[30]Feng X,Iliuk A,Zhang X,et al.Supramolecular exosome array for efficient capture and in situ detection of protein biomarkers[J].Analytical Chemistry,2023,95(5):2812-2821.
[31]Wu T,Liu X,Chen H,et al.An in situ exosomal miRNA sensing biochip based on multi-branched localized catalytic hairpin assembly and photonic crystals[J].Biosensors and Bioelectronics,2023,222:115013.
[32]Chen Y,Zhai L Y,Zhang L M,et al.Breast cancer plasma biopsy by in situ determination of exosomal microRNA-1246 with a molecular beacon[J].Analyst,2021,146(7):2264-2276.
[33]Qian C,Xiao Y,Wang J,et al.Rapid exosomes concentration and in situ detection of exosomal microRNA on agarose-based microfluidic chip[J].Sensors and Actuators B:Chemical,2021,333:129559.
[34]Jiang S,Li Q,Wang C,et al.In situ exosomal microRNA determination by target-triggered SERS and Fe304@TiO2-based exosome accumulation[J].ACS Sensors,2021,6(3):852-862.
[35]Xia Z,Zhang X,Yao J,et al.Giant enhancement of raman scattering by a hollow-core micro structured optical fiber allows single exosome probing[J].ACS Sensors,2023,8(4):1799-1809.
[36]Zhu N,Wang X,Zhang Y,et al.Self-calibrated magnetic aptamer sensor with dual Lanthanideassisted Time-resolved luminescence for high-sensitive detection of melanoma exosomal PD-L1[J].Materials&Design,2023,227:111714.
[37]Wang X,Shepherd S,Li N,et al.A target recycling amplification process for the digital detection of exosomal microRNAs through photonic resonator absorption microscopy[J].Angewandte Chemie International Edition,2023,62(16):e202217932.
[38]Jiang X C,Gao J Q.Exosomes as novel bio-carriers for gene and drug delivery[J].International journal of pharmaceutics,2017,521(1-2):167-175.
[39]Huang L,Rong Y,Tang X,et al.Engineered exosomes as an in situ DC-primed vaccine to boost antitumor immunity in breast cancer[J].Molecular Cancer,2022,21(1):1-19.
[40]Wan T,Zhong J,Pan Q,et al.Exosome-mediated delivery of Cas9 ribonucleoprotein complexes fortissue-specific gene therapy of liver diseases[J].Science Advances,2022,8(37):eabp9435.
[41]Ilahibaks N F,Ardisasmita A I,Xie S,et al.TOP-EVs:Technology of protein delivery through extracellular vesicles is a versatile platform for intracellular protein delivery[J].Journal of Controlled Release,2023,355:579-592.
[42]Huang X,Wu W,Jing D,et al.Engineered exosome as targeted lncRNA MEG3 delivery vehicles for osteosarcoma therapy[J].Journal of Controlled Release,2022,343:107-117.
[43]You Y,Tian Y,Yang Z,et al.Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy[J].Nature Biomedical Engineering,2023,7:887-900.
[44]Zhang C,Wei G,Zhu X,et al.Exosome-delivered circSTAU2 inhibits the progression of gastric cancer by targeting the miR-589/CAPZA1 Axis[J].International Journal of Nanomedicine,2023,18:127-142.
[45]Lu X,Xu G,Lin Z,et al.Engineered exosomes enriched in netrin-1 modRNA promote axonal growth in spinal cord injury by attenuating inflammation and pyroptosis[J].Biomaterials Research,2023,27(1):3.
[46]Hade M D,Suire C N,Suo Z.An effective peptidebased platform for efficient exosomal loading and cellular delivery of a microRNA[J].ACS Applied Materials&Interfaces,2023,15(3):3851-3866.
[47]Sun X,Song W,Teng L,et al.MiRNA 24-3p-rich e xosomes functionalized DEGMA-modified hyaluronic acid hydrogels for corneal epithelial healing[J].Bioactive Materials,2023,25:640-656.
[48]Yuan J,Yang H,Liu C,et al.Microneedle patch loaded with exosomes containing microRNA-29b prevents cardiac fibrosis after myocardial infarction[J].Advanced Healthcare Materials,2023,12(13):2202959.
[49]Li J,Kong J,Ma S,et al.Exosome-coated 10B carbon dots for precise boron neutron capture therapy in a mouse model of glioma in situ[J].Advanced Functional Materials,2021,31(24):2100969.
[50]Li Y,Tian L,Zhao T,et al.A nanotherapeutic system for gastric cancer suppression by synergistic chemotherapy and immunotherapy based on iPSCs and DCs exosomes[J].Cancer Immunology,Immunotherapy,2023,72:1673-1683.
[51]Gong L,Zhou H,Zhang S,et al.CD44-targeting drugdelivery system of exosomes loading forsythiaside a combats liver fibrosis via regulating NLRP3-mediated pyroptosis[J].Advanced Healthcare Materials,2023,12:2202228.
[52]Li Z,Yuan Y,Zhang Z,et al.Cell penetrating peptide modified M2 macrophage derived exo somes treat spinal cord injury and rheumatoid arthritis by loading curcumin[J].Materials&Design,2023,225:111455.
[53]He X,Zhang C,Amirsaadat S,et al.Curcumin-loaded mesenchymal stem cell-derived exosomes efficiently attenuate proliferation and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes[J].Applied Biochemistry and Biotechnology,2023,195(1):51-67.
[54]Si K,Ye Z,Ali D J,et al.Co-delivery of PDL1-blocking scFv and chemotherapeutics using engineered exosomes for cancer therapy[J].Journal of DrugDelivery Science and Technology,2023,82:104337.
[55]Wang C,Li N,Li Y,et al.Engineering a HEK-293T exosome-based delivery platform for efficient tumor-targeting chemotherapy/internal irradiation combination therapy[J].Journal of Nanobiotechnology,2022,20(1):247.
[56]Cui J,Wang X,Li J,et al.Immune exosomes loading self-assembled nanomicelles traverse the blood-brain barrier for chemo-immunotherapy against glioblastoma[J].ACS Nano,2023,17(2):1464-1484.
[57]Zeng J,Sun P,Zhao Y,et al.Bone mesenchymal stem cell-derived exosomes involved co-delivery and synergism effect with icariin via mussel-inspired multifunctional hydrogel for cartilage protection[J].Asian Journal of Pharmaceutical Sciences,2023,18(3):100799.
基本信息:
中图分类号:R341
引用信息:
[1]唐懿柯,谢欣,李达秀.外泌体在疾病诊断和治疗领域的研究进展[J].化学传感器,2024,44(04):23-35.
基金信息:
重庆市教委科学技术研究项目(青年)(KJQN202101136)
2024-12-15
2024-12-15