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miRNA作为一种非编码小分子RNA，在转录后调控基因表达中起着重要作用，其异常表达可诱发多种疾病。大量研究表明，miRNAs的异常表达是由于多种肿瘤类型的基因突变导致，产生的miRNAs也可通过相关基因的调节，进一步促进肿瘤的发展。因此，基于miRNAs的研究是近年的研究热点；其中，靶向miRNAs的诊疗研究对于肿瘤的治疗和研究具有重要意义。此外，近年来，生物正交反应由于其反应的专属性、高效性以及温和性在miRNAs诊疗中取得了重要进展。因此，该文详细总结了生物正交反应的类型、反应机理以及反应条件；在此基础上，进一步讨论了生物正交反应在miRNAs诊疗中的应用，以期为后续miRNAs诊疗研究提供理论和技术支持。
Abstract：MiRNAs, as a small non-coding molecule, play a crucial role in the post-transcriptional regulation of gene expression, and their abnormal expression can lead to many diseases. Numerous studies have demonstrated that gene mutations in various types of tumors can lead to the abnormal expression of miRNAs, which can then be further enhanced by the regulation of related genes to further facilitate tumor progression. Therefore, miRNArelated research has become a popular topic in recent years, especially in terms of diagnosis and treatment. In addition, biorthogonal reactions have made great progress in the diagnosis and treatment of miRNAs due to their specificity, efficiency, and gentleness. This review provides a comprehensive summary of the types, reaction mechanisms, and reaction conditions of biological orthogonal reactions. Furthermore, it discusses the application of biorthogonal reactions in the diagnosis and treatment of miRNAs, in order to provide theoretical and technical support for future miRNA diagnosis and treatment research.
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基本信息:
中图分类号:R313;O657.3
引用信息:
[1]钟敏,周子为,李达秀,等.生物正交反应在microRNAs诊疗中的应用研究[J].化学传感器,2024,44(04):12-22.
基金信息:
重庆市教委科学技术研究项目(青年)(KJQN202201168)
2024-12-15
2024-12-15